<aside> 🚨 Tamoxifen (and derivatives like 4-OHT) is highly carcinogenic so there are special considerations for their mixing and use. Follow these precautions to keep yourself and others safe!

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Table of Contents

Materials & Supplies

• 100% Ethanol
• Cremophor (Sigma C5135-1KG)
• 4-hydroxytamoxifen (Sigma H7904-5MG)
• Sterile vials (1.8mL) or 2.0 mL cyrotubes
• 1.0 mL Eppendorf
• Aluminum foil
• Pipettors
• Pipette tips with filtered tips
• Needles and syringe (for mouse I.P. injections)
• Hot plate
• Vortex

Drug Mixing

<aside> 🚨 Always under a fume hood which must be cleaned with bleach after use.

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1. Mix up fresh 10% bleach solution in a spray bottle (will be effective for 24 hours) or use bleach wipes
2. Gather supplies/materials and label vials