(Adapted from Rothwell Lab protocols)

Table of Contents

Materials & Equipment

  1. Isoflurane
  2. Sterile water
  3. AAV vector to be injected
  4. eye ointment
  5. small mouse fur shaver
  6. stereotaxic with drill, virus injector, and syringe pump controller attachments
  7. alcohol prep pads (70% isopropyl alcohol)
  8. Povidone-Iodine prep pads
  9. Sterile disposable scalpels
  10. Sterile cotton swabs
  11. drill bits
  12. Hamilton gastight syringe (10uL or 50uL)
    1. 10uL is best with the 33G needle for infusion volumes less than 1uL
  13. Hamilton 33G steel needles
  14. ketoprofen
  15. 0.9% Sodium Chloride