Table of Contents

Note: this is the one we use from Morikawa et al.

Materials & Equipment

• Buffers and solutions from IHC Quick Reference
• SuperFrost Plus slide (Fisher Scientific 22-034-979)
• glass dishes/petri dishes to hold brain tissue for mounting
• coverslips (Fisher Scientific 12544E)
• Prolong Diamond Antifade Reagent containing DAPI (Thermo Fisher P36962)
• orbital shaker
• vortex
• aluminum foil
• well plates
• clear nail polish
• fine paint brushes
• Antibodies of interest: Primary and Secondary

Tissue Preparation

Perfusion

1. Follow steps for Mouse Perfusion.