Table of Context

LSM 800 specs of interest for OLRI confocal

ZEISS LSM 800 Overview_Macalester.pptx

• Our auto-standby diode lasers are 405nm, 488nm, 561 nm, 640 nm
  • So try to get antibodies with these 4 fluorophores
• Our detectors:
  • 2 Multiakaline
  • GaAsp (”Channel 2”)
  • ESID - light image
• Our objectives:
  • Air objectives: 5x, 10x, 20x, 40x
  • Oil objectives: 63x

LSM 800 User Quick Guide

Updated Confocal Quick Guide.docx

Table of Contents (For Updated Confocal Quick Guide.docx above)

1. Starting Up the System2
2. Launching the Zen Software3
3. Setting Up the Microscope: Locating Specimens4
4. Loading an Experiment for Routine Confocal Scanning 5
5. Adjusting Channel Sensitivity (“Channels” Window) 6
6. Adjusting Scanning Parameters (“Acquisition Mode” Window)7
7. Setting Up and Acquiring a Z-stack 8
8. Saving Data 9
9. Exporting Data 10