Materials

  1. Mice (CD-1 or C57BL/6)
  2. Distilled water
  3. 12-well plates (10)
  4. 10% Sodium Azide solution in 1X PBS (2L)*
  5. Forceps (20)
  6. Dissecting scissors (20)
  7. Large fabric scissors (2 from Tong Lab)
  8. Razor blades (25; 20 for one lab, 5 for another)
  9. Plastic larger petri dishes (20)
  10. Isoflurane anesthesia (1 full bottle)
  11. 15 mL Eppendorf tubes (20)
  12. Sterile fields (20)
  13. 1X PBS (4L)
  14. Pasteur pipette or 1-5 uL pipette
  15. micropipettes (P5, P10, P200, P1000)
  16. micropipette tips
  17. Orbital shaker at 4*C
  18. Orbital shaker at Room Temperature