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🚨 Tamoxifen (and derivatives) is highly carcinogenic so there are special considerations for their mixing and use. Follow these precautions to keep yourself and others safe!
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TRG_AAV_TAMlogs.docx
Table of Contents
Materials and Reagents:
- Tamoxifen (Sigma‐Aldrich), CAS # 10540‐29‐1
- Corn Oil
- Ethanol (for disinfection)
- 1 ml Syringe (BD)
- 3/8” beveled needle – 26 gauge (BD)
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🖐️ Tamoxifen is a hazardous substance. Review the MSDS, wear appropriate PPE, and, if possible, house mice in disposable pens in a separate animal room for the duration of injections.
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Procedure:
- Determine the injection dose by weight of mouse, using the Castern et al. dosage of 100 mg TAM/ 1 kg mouse.
- Assuming a roughly 20g mouse, this means a mouse receives 2 mg TAM every injection.
- A reasonable single injection volume is 0.5 mL
- Dissolve TAM powder (comes as 1 g or 1000 mg) in 250mL corn oil to reach 2 mg TAM/0.5mL corn oil.
- Dissolve 1 g of Tamoxifen in 250mL corn oil by shaking overnight at 37°C.
- Tamoxifen is light sensitive, and should be made and stored in a light‐ blocking vessel (amber, or foil wrapped).
- After tamoxifen is in solution, store at 4°C for the duration of injections.
- Administer tamoxifen via intraperitoneal injection (using an ACUC approved injection procedure) once every 24 hours for a total of 7 consecutive days.
- As a safeguard, sanitize injection site with 70% ethanol prior to injection.
- Following the final injection, mice should be quarantined for 24 hours before returning to their normal animal room.
- Disposable pens and bedding should be discarded at this time.
- (For Cre characterization work at the Jackson Laboratory, there is a 7‐day waiting period between the final injection and necropsy/histological analysis)
- Throughout the course of tamoxifen injections and any post‐injection wait period, mice should quarantined to another room (animal behaviour suite) and be closely monitored for any adverse reactions to the treatment.
Clean-up & special considerations
- Clean tabletops and other surfaces with fresh 10% bleach solution, followed by water (i.e. stainless steel table tops).