Table of Contents

Lab Schedule (3-hour lab)

1. (20 minutes) Review steps performed by the instructor: Cryoprotection and embedding in O.C.T.
2. (Groups will take turns observing, 15 minutes each, ~ 1 hour total) Observing cryosectioning; observing perfusions
3. (While groups wait) Review your Official Experiment protocol & preparation (Plan out of class dates/times)

TO PREPARE:

• Read and review this lab (there will be many techniques coming up today!)

DUE AFTER LAB:

• Show any of the instruction team your answers to the lab questions. This is comprehension check.
• Submit your Final IHC Protocol via Moodle

Materials & Equipment

• 10% Sodium Azide solution (1 L)
• 0.01% sodium azide solution
• 1 L of 1X PBS
• 6-well plate (5)
• Practice brains (2)
• Cryostat (and accompanying supplies, like O.C.T.)